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Identification, characterization of an AP2/ERF transcription factor that promotes adventitious, lateral root formation in Populus.

Identifieur interne : 002637 ( Main/Exploration ); précédent : 002636; suivant : 002638

Identification, characterization of an AP2/ERF transcription factor that promotes adventitious, lateral root formation in Populus.

Auteurs : Dalila Trupiano [Italie] ; Yordan Yordanov ; Sharon Regan ; Richard Meilan ; Timothy Tschaplinski ; Gabriella Stefania Scippa ; Victor Busov

Source :

RBID : pubmed:23645259

Descripteurs français

English descriptors

Abstract

Using activation tagging in Populus, we have identified five mutant lines showing changes in their adventitious rooting. Among the affected lines, three showed increased and two decreased adventitious rooting. We have positioned the tag in the mutant lines via recovering genomic sequences flanking the left-hand border of the activation tagging vector and validated the transcriptional activation of the proximal genes. We further characterized one line in which the cause of the observed rooting phenotype was up-regulation of a gene encoding a transcription factor of the AP2/ERF family of unknown function (PtaERF003). We show, through retransformation, that this gene has a positive effect on both adventitious and lateral root proliferation. Comparative expression analyses show that the phenotype does not result from ectopic expression but rather up-regulation of the native expression pattern of the gene. PtaERF003 function is linked to auxin signal transduction pathway, as suggested by the rapid induction and accentuated phenotypes of the transgenic plants in presence of the hormone. Upregulation of PtaERF003 led to most significant metabolic changes in the shoot suggesting of a broader regulatory role of the gene that is not restricted to root growth and development. Our study shows that dominant tagging approaches in poplar can successfully identify novel molecular factors controlling adventitious and lateral root formation in woody plants. Such discoveries can lead to technologies that can increase root proliferation and, thus, have significant economic and environmental benefits.

DOI: 10.1007/s00425-013-1890-4
PubMed: 23645259


Affiliations:


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Le document en format XML

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<term>Genes, Dominant (MeSH)</term>
<term>Genotype (MeSH)</term>
<term>Indoleacetic Acids (pharmacology)</term>
<term>Mutation (MeSH)</term>
<term>Phenotype (MeSH)</term>
<term>Plant Growth Regulators (pharmacology)</term>
<term>Plant Leaves (drug effects)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (growth & development)</term>
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<term>Plant Roots (growth & development)</term>
<term>Plant Roots (metabolism)</term>
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<term>Populus (drug effects)</term>
<term>Populus (genetics)</term>
<term>Populus (growth & development)</term>
<term>Populus (metabolism)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Transcription Factor AP-2 (genetics)</term>
<term>Transcription Factor AP-2 (metabolism)</term>
<term>Transcriptional Activation (MeSH)</term>
<term>Up-Regulation (MeSH)</term>
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<term>Acides indolacétiques (pharmacologie)</term>
<term>Activation de la transcription (MeSH)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Facteur de croissance végétal (pharmacologie)</term>
<term>Facteur de transcription AP-2 (génétique)</term>
<term>Facteur de transcription AP-2 (métabolisme)</term>
<term>Feuilles de plante (croissance et développement)</term>
<term>Feuilles de plante (effets des médicaments et des substances chimiques)</term>
<term>Feuilles de plante (génétique)</term>
<term>Feuilles de plante (métabolisme)</term>
<term>Gènes dominants (MeSH)</term>
<term>Génotype (MeSH)</term>
<term>Mutation (MeSH)</term>
<term>Phénotype (MeSH)</term>
<term>Populus (croissance et développement)</term>
<term>Populus (effets des médicaments et des substances chimiques)</term>
<term>Populus (génétique)</term>
<term>Populus (métabolisme)</term>
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<term>Protéines végétales (métabolisme)</term>
<term>Racines de plante (croissance et développement)</term>
<term>Racines de plante (effets des médicaments et des substances chimiques)</term>
<term>Racines de plante (génétique)</term>
<term>Racines de plante (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Régulation positive (MeSH)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
<term>Éthylènes (pharmacologie)</term>
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<term>Transcription Factor AP-2</term>
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<term>Populus</term>
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<term>Plant Roots</term>
<term>Populus</term>
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<term>Facteur de transcription AP-2</term>
<term>Feuilles de plante</term>
<term>Populus</term>
<term>Protéines végétales</term>
<term>Racines de plante</term>
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<term>Plant Roots</term>
<term>Populus</term>
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<term>Facteur de transcription AP-2</term>
<term>Feuilles de plante</term>
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<term>Protéines végétales</term>
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<term>Phénotype</term>
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<div type="abstract" xml:lang="en">Using activation tagging in Populus, we have identified five mutant lines showing changes in their adventitious rooting. Among the affected lines, three showed increased and two decreased adventitious rooting. We have positioned the tag in the mutant lines via recovering genomic sequences flanking the left-hand border of the activation tagging vector and validated the transcriptional activation of the proximal genes. We further characterized one line in which the cause of the observed rooting phenotype was up-regulation of a gene encoding a transcription factor of the AP2/ERF family of unknown function (PtaERF003). We show, through retransformation, that this gene has a positive effect on both adventitious and lateral root proliferation. Comparative expression analyses show that the phenotype does not result from ectopic expression but rather up-regulation of the native expression pattern of the gene. PtaERF003 function is linked to auxin signal transduction pathway, as suggested by the rapid induction and accentuated phenotypes of the transgenic plants in presence of the hormone. Upregulation of PtaERF003 led to most significant metabolic changes in the shoot suggesting of a broader regulatory role of the gene that is not restricted to root growth and development. Our study shows that dominant tagging approaches in poplar can successfully identify novel molecular factors controlling adventitious and lateral root formation in woody plants. Such discoveries can lead to technologies that can increase root proliferation and, thus, have significant economic and environmental benefits. </div>
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